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. Author manuscript; available in PMC: 2024 Mar 25.
Published in final edited form as: Science. 2023 Jul 27;381(6656):420–427. doi: 10.1126/science.adi1024

Fig. 2. CF multi-innervation of mature multibranched PCs.

Fig. 2.

(A) Schematic of tracer (DA-594) injection. (B) A Poly PC after immunolabeling for PCs (calbindin) and CF terminals (VGluT2). The tracer label distinguishes CFs with distinct olivary origin on the left and right trunks. (C) Scheme of whole-cell patch-clamp in cerebellar slices and CF EPSCs recorded from either a mono- or multi-innervated PC. (D) Number of mono- versus multi-innervated PCs as a combined population (left). Categorizing by morphology reveals that effectively all multi-innervation occurs in multibranched PCs (n = 50 animals, 159 cells). (E) Summed multi-CF EPSCs are larger than mono-CF EPSCs (n = 135 and 24 cells). The weaker of multiple CFs typically provides >200 pA signals. Holding potential: −10 to −30mV (n = 24 cells). (F) Multi-innervated PCs have earlier dendrite bifurcations (n = 135 and 24 cells). (G) Among PCs with a bifurcated primary dendrite, multi-innervated cells have a wider distance between compartments (n = 85 and 9 cells). (H) Multi-innervated Poly PCs have a wider angle between emerging trunks (n = 26 and 8 cells). Summary points indicate mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.