Table 1.

Mass spectrometry conditions of eight analytes and two ISs.

Analytes	Ion mode	Q1 (m/z)	Q3 (m/z)	DP (V)	CE (V)	CXP (V)
Chlorogenic acid	[M–H]−	353.0	a191.0	−80	−25	−1
			^ 135.0	−80	−29	−10
Hydroxysafflor yellow A	[M–H]−	611.0	a491.0	−100	−44	−1
			^ 403.1	−100	−46	−17
Hyperoside	[M–H]−	462.9	a299.9	−75	−32	−31
			^ 271.2	−75	−64	−5
Kaempferol-3-O-glucoside	[M–H]−	446.9	a283.8	−90	−36	−11
			^ 227.0	−90	−70	−7
Kaempferol-3-O-rutinoside	[M–H]−	593.0	a284.8	−80	−38	−19
			^ 254.9	−80	−62	−9
N-acetyl-L-glutamine	[M–H]−	186.9	a144.9	−75	−18	−9
			^ 127.1	−75	−14	−7
p-Coumaric acid	[M–H]−	163.1	a93.0	−80	−36	−12
			^ 116.7	−80	−42	−9
Rutin	[M–H]−	609.2	a300.0	−150	−51	−17
			^ 271.0	−150	−64	−21
Icariin (IS)	[M-H-Glc]−	513.1	a366.1	−120	−36	−25
			^ 323.0	−120	−52	−21
N-carbamoyl-L-glutamate (IS)	[M–H]−	188.9	a146.1	−80	−16	−9
			^ 101.7	−80	−26	−7

^aThe ion pairs adopted for quantification,

^{^}the ion pairs employed for qualitation.