Supplementary Table 4.

Results of freeze-thaw stability (three times) of all analytes (n = 6).

Analytes	Concentration (ng/mL)	Mean ± SD	RSD	RE
Chlorogenic acid	5	4.527 ± 0.130	2.86%	−9.46%
	50	50.717 ± 1.737	3.42%	1.43%
	500	483.647 ± 9.298	1.92%	−3.27%
Hydroxysafflor yellow A	5	5.115 ± 0.341	6.66%	2.30%
	50	50.550 ± 1.276	2.52%	1.10%
	500	508.052 ± 21.065	4.15%	1.61%
Hyperoside	5	5.384 ± 0.142	2.64%	7.69%
	50	51.911 ± 1.743	3.36%	3.82%
	500	488.61 ± 16.242	3.33%	−2.37%
Kaempferol-3-O-glucoside	5	5.390 ± 0.067	1.24%	7.80%
	50	53.303 ± 1.079	2.02%	6.61%
	500	524.815 ± 22.328	4.25%	4.96%
Kaempferol-3-O-rutinoside	5	5.479 ± 0.134	2.45%	9.58%
	50	52.642 ± 0.771	1.46%	5.28%
	500	539.455 ± 23.696	4.39%	7.89%
N-acetyl-L-glutamine	5	5.109 ± 0.257	5.03%	2.18%
	50	50.906 ± 3.522	6.92%	1.81%
	500	527.696 ± 15.640	2.96%	5.54%
p-Coumaric acid	5	4.891 ± 0.196	4.01%	−2.18%
	50	53.579 ± 2.574	4.80%	7.16%
	500	510.543 ± 9.094	1.78%	2.11%
Rutin	5	5.067 ± 0.211	4.17%	1.35%
	50	53.579 ± 2.574	4.80%	7.16%
	500	548.234 ± 7.459	1.36%	9.65%