Suppl. Table 3.

Intra- and inter-day precision, accuracy of the eleven analytes in rat plasma. (n = 6)

Components	Spiked concentration (ng/mL)	Intra-day		Inter-day
		Precision	Accuracy	Precision	Accuracy
		RSD (%)	RE (%)	RSD (%)	RE (%)
Calycosin	0.5	12.41	−2.00	7.31	−1.56
	1.5	4.08	−0.70	4.44	−0.22
	100	11.57	−1.28	9.14	1.16
	2000	3.54	−6.41	5.83	−4.33
Calycosin-7-O-β-D-glucoside	3.5	3.31	4.40	3.72	2.51
	10.5	8.56	−0.04	9.12	0.86
	100	5.64	8.17	9.40	2.31
	6000	2.63	4.60	5.72	−1.16
Formononetin	8	8.06	−10.83	8.51	−10.60
	24	4.60	−6.82	10.33	2.44
	100	10.56	6.27	9.92	2.08
	2000	11.51	8.45	7.91	3.53
Ononin	1	5.96	−7.67	7.66	−11.89
	3	5.69	−13.67	10.94	−8.48
	100	4.55	12.52	9.58	3.78
	2000	2.29	−6.29	6.73	−1.47
Isomucronulatol 7-O-glucoside	1	4.75	−12.33	6.97	−13.33
	3	5.16	9.89	8.32	1.41
	100	10.37	4.95	7.46	7.39
	2000	1.76	−4.22	5.31	−1.12
9,10-dimethoxyptercarpan-3-O-β-D-glucoside	10	7.26	11.23	7.41	11.42
	30	3.08	10.67	5.36	10.63
	100	5.54	9.00	7.35	10.57
	2000	3.86	12.50	3.62	12.57
Astragaloside IV	5	3.50	−5.30	4.36	−4.53
	15	5.08	−4.00	4.06	−5.99
	100	14.58	3.85	12.26	4.32
	4000	2.05	8.90	4.62	8.66
Astragaloside III	10	2.28	9.90	8.27	10.24
	30	3.08	7.80	4.86	11.17
	100	8.75	3.57	8.75	7.08
	2000	8.65	6.32	8.65	3.08
Hydroxysafflor yellow A	8	5.52	−5.80	4.24	−6.31
	24	6.83	8.58	5.10	9.83
	2000	5.41	−5.65	3.85	−4.12
	30000	2.95	8.18	5.12	12.66
Anhydrosafflor yellow B	50	2.34	6.69	4.58	10.01
	150	3.43	10.69	4.96	13.51
	2000	1.90	8.50	7.43	−0.18
	20000	4.18	13.07	5.88	12.66
Carthamin	50	2.68	−6.84	3.91	−7.08
	150	3.22	−8.18	5.88	−5.85
	2000	12.31	6.23	10.09	9.48
	10000	12.74	11.60	8.55	12.44