Suppl. Table 4.

Stability of the eleven analytes in rat plasma. (n = 6)

Components	Spiked concentration (ng/mL)	room temperature for 12 h (RSD%)	4 °C for 24 h (RSD%)	−20 °C for 7 days (RSD%)	After three freezes (at −20 °C)/thaw (at 4 °C) cycle (RSD%)
Calycosin	1.50	7.34	3.38	3.64	8.13
	100	8.93	6.25	6.30	5.73
	2000	9.08	3.69	7.57	6.05
Calycosin-7-O-β-D-glucoside	10.5	6.40	3.89	7.71	10.83
	100	8.67	5.11	4.04	11.20
	6000	5.28	4.81	3.71	3.56
Formononetin	24	5.04	11.81	4.35	7.69
	100	4.49	6.86	4.80	8.05
	2000	2.89	6.24	6.61	6.90
Ononin	3	4.99	3.48	7.45	4.80
	100	9.65	7.53	8.00	11.50
	2000	6.69	7.48	8.05	7.66
Isomucronulatol 7-O-glucoside	3	7.45	8.06	6.97	12.89
	100	7.23	7.94	9.43	9.11
	2000	2.62	7.00	6.53	8.75
9,10-dimethoxyptercarpan-3-O-β-D-glucoside	30	4.48	5.90	7.84	7.79
	100	10.44	11.04	13.51	9.44
	2000	8.54	14.92	1.95	10.05
Astragaloside IV	15	10.93	3.19	5.37	8.11
	100	12.27	6.07	10.12	5.96
	4000	5.11	8.00	7.93	8.09
Astragaloside III	30	6.78	10.75	10.59	11.24
	100	12.27	8.69	8.41	12.48
	2000	14.91	6.80	5.07	7.11
Hydroxysafflor yellow A	24	9.90	6.30	9.07	7.49
	2000	6.25	5.54	8.51	9.39
	30000	6.23	4.50	6.21	6.33
Anhydrosafflor yellow B	150	2.40	8.98	10.04	9.09
	2000	9.53	7.35	9.81	10.05
	20000	9.69	8.32	8.39	9.18
Carthamin	150	12.64	13.11	13.87	12.11
	2000	7.69	10.81	11.67	11.77
	10000	10.27	13.94	14.33	13.33