Suppl. Table 5.

Recovery and matrix effect of the thirteen analytes in rat plasma. (n = 6)

Components	Spiked concentration (ng/mL)	Recovery	Matrix effect
		(Mean ± SD) %	(RSD) %
Calycosin	1.5	86.4 ± 4.3	1.08
	100	85.8 ± 9.2	2.93
	2000	86.5 ± 7.1	2.06
Calycosin-7-O-β-D-glucoside	10.5	84.8 ± 10.0	3.44
	100	89.8 ± 3.7	2.38
	6000	83.2 ± 11.6	2.61
Formononetin	24	87.7 ± 2.6	5.39
	100	87.6 ± 9.2	2.41
	2000	88.5 ± 11.7	1.95
Ononin	3	84.0 ± 4.4	1.98
	100	90.5 ± 9.8	4.46
	2000	87.3 ± 8.5	3.26
Isomucronulatol 7-O-glucoside	3	86.1 ± 10.0	2.21
	100	86.7 ± 5.5	1.17
	2000	85.3 ± 9.2	3.99
9,10-dimethoxyptercarpan-3-O-β-D-glucoside	30	79.0 ± 1.8	1.93
	100	80.8 ± 4.5	5.32
	2000	78.8 ± 10.2	1.29
Astragaloside IV	15	85.7 ± 12.4	6.38
	100	86.5 ± 6.2	6.22
	4000	85.2 ± 5.4	4.85
Astragaloside III	30	85.5 ± 6.9	3.98
	100	85.2 ± 3.0	7.28
	2000	88.5 ± 8.9	3.15
Hydroxysafflor yellow A	24	86.0 ± 12.6	6.35
	2000	88.5 ± 12.7	7.91
	30000	82.0 ± 11.1	7.97
Anhydrosafflor yellow B	150	85.3 ± 2.0	7.30
	2000	85.6 ± 7.3	7.87
	20000	81.0 ± 9.9	14.68
Carthamin	150	79.5 ± 5.3	10.98
	2000	86.1 ± 8.1	2.13
	10000	77.9 ± 4.1	7.37
Digoxin	1000	87.9 ± 8.3	13.28
Puerarin	100	85.5 ± 9.1	1.96