TABLE 5.

HLA restriction of recognition of dengue virus proteins by CTL lines of patients KPP94-037 and KPP94-024^a

Patient KPP94-037b													Patient KPP94-024c
Expt 1					Expt 2				Expt 3				Expt 4				Expt 5
BLCL	HLA class I	% Specific 51Cr release by indicated T-cell line			BLCL	HLA class II	% Specific 51Cr release by indicated T-cell line		BLCL	HLA class II	% Specific 51Cr release by indicated T-cell line		BLCL	HLA class I	% Specific 51Cr release by indicated T-cell line		BLCL	HLA class I	% Specific 51Cr release by indicated T-cell line
		3E7	2E4	2C8			2D11	2G3			2D11	2G2			2F5	2C8			2F5	3C2	3B11
Autologous	A1,11.1 B46,57 C1,6	95	91	79	Autologous	DP5 DQ9 DR7,9	56	55	Autologous	DP5 DQ9 DR7,9	71	39	Autologous	A2,24 B7,46 C1,7	70	68	Autologous	A2,24 B7,46 C1,7	78	75	82
HCV57	A2.1,3 B7,37 C6,7	11	−4	−4	9077	DP5 DQ3 DR9,12	−3	−7	KPP94-024	DP5 DQ9 DR9,12	−3	1	VA03	A2,24 B7,35 C3,7	32	23	Tom G	A23,29 B7,44 C4	28	56	51
9052	A1,3 B27,57 C2,6	29	22	21	CP	DP4 DQ2,3 DR5,7	30	30	CHD94-134	DP4 DQ9,7 DR7,12	78	34	9077	A2 B46 C11	−5	−5	GM3161	A3,3 B7,7	41	44	46
GM3098A	A2,2 B 57,57	71	51	53					MS	DQ1,3 DR1,4		−7	CP	A2,28 B51,57 C6	−4	0	GM6825A	A23 B7,7	26	53	52

^aAllogeneic BLCLs that shared one or more HLA class I or class II alleles with the autologous line, as indicated, were used as targets in the assays. The E/T ratios were between 16:1 and 40:1 for all T-cell lines used. 

^bTarget cells were infected with Vac.D2NS1.2a. 

^cTarget cells were infected with Vac.D2NS3.