Figure 3: ERACR construct designs and crossing schemes.

A) Diagrams of MCR-GFP and ERACR elements. Fly heads on the left indicate the phenotype of each strain. Schematic not drawn to scale. B) Cross schemes to generate F₁ “master females” with ERACRs in-trans to the MCR-GFP element and their F₂ progeny. Crossing schemes for y⁻ ERACR-min (left) and for y⁺ ERACR-1 and y⁺ ERACR-2 (right). Fly heads depict eye color (wild-type = w⁺ (red), w⁻ (white), or w^a (orange), eye fluorescence (radial emanating lines of eGFP (green), DsRed (red), both eGFP and DsRed (alternating green and red), or neither fluorescence (white), and body color: y⁺ (brown) or y⁻ (yellow). Expected F₂ phenotypes bolded and outlined with black boxes.