Figure 4: ERACRs delete and replace the MCR-GFP drive.

Phenotypic frequencies and deduced gene conversion events in F₂ progeny from crosses depicted in Fig. 3A. (additional crossing schemes in Figs. S13A–E). Black type = mean of percentages across all vials and orange type = estimated receiver conversion frequencies (e.g., DsRed w^a males/total w^a males) (see Data S3 for details). A) DsRed⁺ inheritance is a proxy for scoring ERACR prevalence (DsRed⁺, GFP⁻ and DsRed⁺, GFP⁺ progeny included). A’) The subset of data plotted in panel 4A with traceable donor and receiver chromosomes re-plotted by donor (w⁻; left graph) versus receiver (w^a; right graph) chromosomes. B) Proportion of F₂ males or females inheriting the donor (w⁻) versus receiver (w^a) chromosome. C,E) Schematics illustrating predicted gene conversion events responsible for specific phenotypes: sequences of relevant junctions shown in (Fig. S14). D) GFP inheritance is a proxy for MCR-GFP prevalence (DsRed⁺,GFP⁺ plus DsRed⁻,GFP⁺ F₂ progeny). E) MCR-GFP alleles, although intact, have NHEJ-induced indels at the y2 and y3 cut sites.