Table 2. Results of PCR amplification and sequencing of 18S rRNA genes in nutria feces using previously reported primers.
| Primers |
Samples |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Forward | Reverse | Expected size (bp) | A |
B |
C |
||||||
| Expected*1 amplicon | Sequences*2 | Nonspecific*3 bands | Expected*1 amplicon | Sequences*2 | Nonspecific*3 bands | Expected*1 amplicon | Sequences*2 | Nonspecific*3 bands | |||
| Nem18SF | 18SPCR | 1,500 | - | / | +++ | - | / | ++ | + | - | +++ |
| 18SP2F | 18SPCR | 950 | + | - | +++ | + | - | +++ | + | - | +++ |
| SSU24HF | 18SPCR | 750 | + | Sm | +++ | - | / | +++ | + | Sm | +++ |
| 18SP4F | 18SPCR | 710 | + | Sm | +++ | + | - | +++ | + | Sm | +++ |
| SSU23F | 18SPCR | 320 | + | - | +++ | + | - | ++ | + | - | +++ |
| Nem18SF | 18SP8R | 1,000 | + | Sm | ++ | - | / | + | + | Sm | ++ |
| 18SP2F | 18SP8R | 550 | + | Sm | ++ | + | - | ++ | + | N.D.*4 | ++ |
| SSU24HF | 18SP8R | 250 | + | - | - | + | - | ++ | + | - | + |
| 18SP4F | 18SP8R | 210 | + | - | - | + | - | - | + | - | + |
*1 Expected amplicon. + and -: a band of the expected size was or was not present, respectively. *2 Sequences. /: not done, Sm: identical to those of S. myopotami; -: not determined due to multiple sequence signals or high homology with other microorganisms. *3 Nonspecific bands. -: 0 bands; +: 1 band; ++: 2 to 5 bands; +++: more than 6 bands. *4 N.D.: not determined due to the presence of 2 to 5 nonspecific bands around the expected amplicon.