Figure 2.

Tissue microarray (TMA) analysis of LARP4A and LARP4B expression in human osteosarcoma and prostate adenocarcinoma

(A and B) Immunohistochemical analysis of LARP4A and LARP4B protein expression in representative core samples of (A) osteosarcoma and (B) prostate adenocarcinoma biopsies showed high expression of each LARP4 paralog in respective tumor tissues (Cancer) compared to Normal samples. Scale bars, 0.5mm.

(C) Histological analysis of representative human osteosarcoma sections showing little to no expression of either LARP4A (C(i)) or LARP4B (C(iii) in normal osteoblasts (arrows) lining bone (B), and markedly higher expression in tumor tissue (C(ii,iv)) particularly in transformed osteoblasts (arrows). Insets in C(ii) and C(iv) depict the nuclear localization of LARP4A and LARP4B, respectively (arrows). Scale bars, 50um; insets 25um.

(D) Histological analysis of representative prostate adenocarcinoma sections showing little to no expression of either LARP4A D(i) or LARP4B (iii) in normal prostate tissue, and markedly high expression in transformed prostate epithelial cells in tumor tissue (D(ii,iv). Arrows in D(iv) and inset depict the nuclear localization of LARP4B. Scale bars, 25um.

(E and F) Quantification of LARP4A and LARP4B expression in (E) osteosarcoma and (F) prostate adenocarcinoma TMAs. The data represent the mean (±SEM) area of DAB-positive staining following LARP4A/LARP4B staining in normal and cancer tissues relative to the overall core area. For osteosarcoma, n = 11 duplicate cores for normal bone, n = 81 duplicate cores for cancer tissue, areas of bone marrow was excluded from analyses. For prostate adenocarcinoma, n = 12 duplicate cores for each normal and cancer tissues. T-tests were used to test for statistical significance between normal vs. cancerous tissues for each cancer type and LARP4 stain. ∗p < 0.05; ∗∗∗∗p < 0.0001.