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. 2024 Feb 24;27(4):109288. doi: 10.1016/j.isci.2024.109288

Figure 5.

Figure 5

LARP4A and LARP4B regulate cell proliferation and LARP4B affects cell cycle progression

(A‒C) MTS viability assays of PC3 (A), MG63 (B) and MNNG/HOS (C) control cells (non-transfected NTC, siCon) and siLARP4A or siLARP4B knockdown cells over a 4days time course. The data represent the mean ± SEM, n = 8 from 4 independent experiments. p values are indicated (light gray value indicates the p value of the siControl vs. siLARP4A comparison; black of the siControl vs. siLARP4B comparison).

(D) Quantification of PC3 (left) and MG63 (right) cell numbers after 72h following knockdown of siLARP4A (si4A1, si4A2), and siLARP4B (si4B1, si4B2) normalized to non-targeting siRNA (siCon) controls. NTC – non-transfected controls. The data represent the mean ± SEM, n = 11 from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

(E and F) Immunofluorescence images of siControl, siLARP4A, and siLARP4B knockdown PC3 (E) and MG63 (F) cells following EdU incorporation and pHH3 staining. Hoechst was used as a counterstain. Scale bars, 200μm.

(G and H) Multiparametric cell cycle analysis of PC3 (G) and MG63 (H) cells either non-transfected (NTC) or transfected with non-targeting control (siCon) and siRNAs targeting LARP4A (si4A1, si4A2), LARP4B (si4B1, si4B2) as indicated. The fraction of the population at each cell cycle stage (sub-G1, G1, S, G2, and M phases) for each cell type is depicted. The data represent the mean ± SEM, n = 12 from four independent experiments. p values are indicated. See also Figure S5.