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. 2024 Feb 24;27(4):109288. doi: 10.1016/j.isci.2024.109288

Figure 8.

Figure 8

LARP4A and LARP4B regulate cell morphology

(A and B) Immunofluorescence images of PC3 (A) or MG63 (B) control (NTC, siControl) and LARP4A (LARP4Asi1, LARP4Asi2) or LARP4B (LARP4Bsi1, LARP4Bsi2) knockdown cells stained with phalloidin and DAPI for morphological analyses. Scale bars, 50μm.

(C‒F) Morphological analysis of PC3 (C and D) and MG63 (E and F) cell perimeter and circularity as indicated. For all violin plots, the line depicts the mean, n = 178–220 cells from 3 independent experiments. p values are indicated.

(G) Immunofluorescence images of PC3 cells overexpressing GFP-LARP4A, GFP-LARP4B, or empty GFP plasmid. Scale bars, 100μm.

(H and I) Cell area (H) and circularity (I) of PC3 cells overexpressing LARP4A and LARP4B versus an empty plasmid control. Lines in violin plots depict the mean, n = 600–1000 GFP+ cells from 3 independent experiments.

(J) Immunofluorescence images of MG63 cells overexpressing GFP-LARP4A, GFP-LARP4B, or empty GFP plasmid. Scale bars, 100μm.

(K and L) Cell area (K) and circularity (L) of MG63 cells overexpressing LARP4A and LARP4B versus an empty plasmid control. Lines in violin plots depict the mean, n = 600–1000 GFP+ cells from 3 independent experiments. p values are indicated. See also Figure S10.