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. 2024 Feb 24;27(4):109288. doi: 10.1016/j.isci.2024.109288

Figure 9.

Figure 9

LARP4A and LARP4B regulate cell migration and invasion

(A and B) Wound closure assays in PC3 (A) and MG63 (B) cells over 24-48h either non-transfected (NTC) or transfected with non-targeting control (siCon) and siRNAs targeting LARP4A (si4A1, si4A2) or LARP4B (si4B1, si4B2) as indicated. Blue lines depict the area of the wound at t = 0h and black/white image shows the mask used for wound area analysis. Scale bars, 200μm.

(C and D) Quantification of would area closure by PC3 (C) and MG63 (D) cells over the experimental time course shown in (A and B). The data indicate the mean ± SEM, n = 12–22 regions of interest from 3 independent experiments. p values are indicated.

(E and F) Individual migration tracks of 20 randomly selected PC3 (E) and MG63 (F) control (siControl), siLARP4A, or siLARP4B knockdown cell tracks during a 16h time lapse experiment.

(G and H) Migration analysis (velocity, accumulated distance and Euclidean distance) following the quantification of cell tracks from PC3 (G) and MG63 (H) cell either non-transfected (NTC, siCon) or transfected with siRNAs targeting LARP4A (si4A1, si4A2) or LARP4B (si4B1, si4B2). The data represent the mean ± SEM, n = 65–180 cells from 3 independent experiments. p values are indicated.

(I and J) Brightfield images of transwells stained with crystal violet following a 24h migration assay of PC3 (I) and MG63 (J) control (siControl), siLARP4A (4A1, 4A2), or siLARP4B (4B1, 4B2) knockdown cells. Scale bars, 200μm.

(K and L) Quantification of migrated PC3 (K) and MG63 (L) cells over 24h of either non-transfected cells (NTC, siCon) or cells transfected with siRNAs targeting LARP4A (si4A1, si4A2) or LARP4B (si4B1, si4B2). The data represent the mean ± SEM, n = 15 from 3 independent experiments. p values are indicated. See also Figure S11 and Videos S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, and S12.