REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-LARP4A	Abcam	ab241489; RRID: AB_3094705
Rabbit anti-LARP4A	Merck	HPA039306; RRID: AB_3094712
Rabbit anti-LARP4B	Abcam	ab197085; RRID: AB_3094706
Rabbit anti-LARP4B	Merck	HPA036566; RRID: AB_10673624
Rabbit anti-GAPDH	Cell Signalling Technology	5174; RRID: AB_10622025
Rabbit α-tubulin	Cell Signalling Technology	2144; RRID: AB_2210548
Mouse anti-E-cadherin	Abcam	ab76055; RRID: AB_1310159
Rabbit anti-Vimentin	Cell Signalling Technology	5741; RRID: AB_10695459
Mouse anti-TOM20	Santa Cruz Biotechnology	sc17764; RRID: AB_628381
Mouse Lamin A/C	Santa Cruz Biotechnology	sc7292; RRID: AB_627875
Rabbit anti-E2F1	Cell Signalling Technology	3742; RRID: AB_2096936
Rabbit anti-Aurora B	Abcam	ab2254; RRID: AB_302923
Rabbit anti-Cyclin B1	Cell Signalling Technology	4138; RRID: AB_2072132
Rabbit anti-Cyclin E2	Cell Signalling Technology	4132; RRID: AB_2071197
Goat anti-rabbit HRP-conjugated IgG	Jackson Immunoresearch	111-035-003; RRID: AB_2313567
Horse anti-mouse HRP-conjugated IgG	Cell Signalling Technology	7076; RRID: AB_330924
Rabbit anti-Ki67	Abcam	ab15580; RRID: AB_443209
Chicken anti-GFP	Abcam	ab13970; RRID: AB_300798
Rabbit anti-Cleaved Caspase 3	Cell Signalling Technology	9661; RRID: AB_2341188
Rabbit anti-pHH3	Cell Signalling Technology	9701; RRID: AB_331535
Goat anti-Rabbit AlexaFluor 488	Invitrogen	A11034; RRID: AB_2576217
Goat anti-Rabbit AlexaFluor 546	Invitrogen	A11035; RRID: AB_2534093
Goat anti-Chick AlexaFluor 647	Abcam	ab150171; RRID: AB_2921318
Goat anti-mouse AlexaFluor 647	Invitrogen	A21236; RRID: AB_2535805
Bacterial and virus strains
XL-10 Gold Ultracompetent cells	Agilent	200314
Biological samples
Prostate cancer tissue array	Insight Bio	PR481
Bone and cartilage tumour tissue array	Insight Bio	OS804d
Normal bone tissue array	Insight Bio	BO244g
Chemicals, peptides, and recombinant proteins
Lipofectamine 2000	Invitrogen	11668027
Lipofectamine RNAiMAX	Invitrogen	13778100
OptiMEM serum-free medium	Gibco	31985062
mmLVRT reverse transcriptase	Promega	M1701
Brilliant II SYBR Green QPCR Master Mix	Agilent	600828
Laemmli buffer	Bio-Rad	1610747
2-mercaptoethanol	Merck	M3701
Clarity ECL substrates	Bio-Rad	1705060
Fluoroshield with DAPI	Abcam	Ab104139
DAB subtrate kit	VectorLabs	SK-4100
ProLong Gold Antifade Mountant	Invitrogen	P36930
Phalloidin-TRITC	Merck	P1951
Annexin V AlexaFluore 350 conjugate	Invitrogen	A23202
Propridium Iodide	Invitrogen	P3566
Matrigel	Corning	354277
Xenolight D-luciferin substrate	PerkinElmer	122799
CellTrackerTM Green CMFDA Dye	Invitrogen	C2925
Critical commercial assays
RNeasy Mini kit	Qiagen	74004
Bicinchoninic acid standard curve assay	Pierce	23225
Standard cell fractionation kit	Abcam	ab109719
AlexaFluor 647 Click-iT EdU Proliferation kit	Invitrogen	C10340
CellTitre 96 AQueous One Solution MTS	Promega	G3582
Deposited data
Raw bulk RNAseq data	This paper	GEO: GSE222467
Experimental models: Cell lines
Human: MG63 osteosarcoma	ATCC	CRL-1427
Human: PC3 prostate cancer	Dr. Magali Williamson, King’s College London	CRL-1435
Human: MNNG/HOS osteosarcoma +GFP/Luc reporter	From Tattersall et al.51	N/A
Experimental models: Organisms/strains
Mouse: CB17/lcr-Prkdcscid/lcrlcoCrl	Charles River Laboratories	N/A
Mouse: Balb/c Nude	Charles River Laboratories	N/A
Oligonucleotides
ON-TARGETplus Non-targeting siRNA #2	Horizon Discovery	D-001810-02
siGENOME Human LARP4 siRNA	Horizon Discovery	D-016523-02
siGENOME Human LARP4 siRNA	Horizon Discovery	D-016523-04
siGENOME Human LARP4B siRNA	Horizon Discovery	D-026388-01
siGENOME Human LARP4B siRNA	Horizon Discovery	D-026388-04
Recombinant DNA
pCB6-GFP plasmid	Laboratory of Anne Ridley	N/A
pCB6-GFP-LARP4A	This paper	N/A
pCB6-GFP-LARP4B	This paper	N/A
pCMV2-FLAG-LARP4A	From Yang et al.24	N/A
Software and algorithms
ImageLab	Bio-Rad	N/A
QuPath	From Bankhead et al.79	N/A
ImageJ	National Institute of Health	N/A
JACoP	From Bolte et al.80 and Schneider et al.81	N/A
Harmony with PhenoLOGIC	PerkinElmer	N/A
MetaMorph	Molecular Devices	N/A
Chemotaxis and Migration tool	Ibidi	N/A
SkyScan NRecon software v.1.6.9	Bruker	N/A
FastQC	From Andrews82	N/A
HISAT2	From Kim et al.83	N/A
featureCounts	From Liao et al.84	N/A
EdgeR	From Liu et al.85 and Robinson et al.86	N/A
Enrichr	From Chen et al.87	N/A
Prism	GraphPad	N/A
Other
NuPAGE 4-12% Bis-Tris gels	Invitrogen	NP0321BOX
MES running buffer	Invitrogen	NP000202
8μm pore Transwell chambers	Thermo Scientific	140656