Extended Data Fig. 3. The cooperative regulation of food intake from the interplay of AgRP and POMC neurocircuits is also present in female mice.
a, Cumulative food intake for 24h in female mice treated with vehicle and CNO in a cross-over experimental design ***P = 0.0002 AgRP-Gq, **P = 0.0042 POMC-Gi and **P = 0.0031 AgRP-Gq;POMC-Gi, Veh vs. CNO, respectively. b, Quantification of delta food intake (∆ = CNO - Vehicle) during the indicated time intervals after CNO or vehicle injection in female mice 4h: **P = 0.0019 AgRP-Gq, *P = 0.0375 AgRP-Gq;POMC-Gi; Light cycle: ***P = 0.00008 AgRP-Gq, *P = 0.0269 POMC-Gi, **P = 0.0024 AgRP-Gq;POMC-Gi. c, Average of respiratory exchange ratio (RER) during light and dark cycles in female mice treated with vehicle or CNO.***P = 0.0002 AgRP-Gq, *P = 0.0113 AgRP-Gq;POMC-Gi, Veh vs. CNO. d, Curves depicting the changes in RER after injection of vehicle or CNO during the light cycle in male (upper) or female (lower) mice. e, Curves showing no differences in EE in experimental male and female mice treated with CNO or vehicle. f, Curves showing no changes between experimental groups in the total activity curves of male and female mice after CNO or vehicle injection. Data showed the mean ± s.e.m. for each group, with n = 11 control, 13 AgRP-Gq, 12 POMC-Gi and 12 AgRP-Gq;POMC-Gi female mice in a – c and lower panels d – f; and n = 11 control, 10 AgRP-Gq, 12 POMC-Gi, 13 AgRP-Gi;POMC-Gq male mice in upper panel d – f. Statistical test: 1-way ANOVA followed by Tukey multiple comparison test for b, c; 2-way ANOVA followed by Bonferroni′s multiple comparisons test for a, and 2-way ANOVA Mixed effects model for d – f. Source data and further details of statistical analyses are provided as a Source Data file.