Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 12;15:1334762. doi: 10.3389/fimmu.2024.1334762

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Salerno-Goncalves, Chen, Bafford, Izquierdo, Hormazábal, Lagos, Tettelin, D’Mello, Booth, Fasano, Levine and Sztein

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Salmonella modulation of gallbladder epithelial cell molecular pathways. Epithelial cells from HODGM model were exposed to 13 S. Typhi strains isolated from acutely infected (n=6, ) or chronically (n=7, ) infected individuals. HODGM models cultured with media only were used as negative controls. After 5 hours, the cells were harvested, lysed, and phosphorylation of (A) AKT, (B) YAP, (C) mTOR, (D) MAPK, and (E) S6 were detected by western blot. Data are representative of the net responses observed in at least 4 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed to S. Typhi. Each dot is the average of 2 technical independent replicates. The density was normalized to the corresponding pan kinase. Two-tailed nested-t-tests were used to account for the repeated measures within the groups. Dotted lines represent the mean responses. (F) Shown is a representative immunoblot analysis of protein lysates. Results from uninfected media controls (UN) and acute and chronic strains are displayed (G) Correlation between phosphorylation levels of S6 and MAPK using the combined data after exposure to S. Typhi strains isolated from acutely and chronically infected individuals. The solid line represents the trendline. Dashed lines represent 95% confidence intervals. Shown are the coefficient of determination “r” and the “P” value. Correlations used the two-sided Pearson Product Moment tests. (H-J) MAPK regulated transcription factors (H) Phospho-ATF-2, (I) Phospho-MEF2, and (J) Phospho-c-Myc were detected by ELISA-based assays, TransAM assays. Data are representative of the net responses observed in 5 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed S. Typhi. Each dot is the average of 2 technical independent replicates. Two-tailed nested-t-test was used to account for the repeated measures within the groups. P values < 0.05 were considered statistically significant. Dotted lines represent the mean responses.

Inline graphic — Salmonella modulation of gallbladder epithelial cell molecular pathways. Epithelial cells from HODGM model were exposed to 13 S. Typhi strains isolated from acutely infected (n=6, ) or chronically (n=7, ) infected individuals. HODGM models cultured with media only were used as negative controls. After 5 hours, the cells were harvested, lysed, and phosphorylation of (A) AKT, (B) YAP, (C) mTOR, (D) MAPK, and (E) S6 were detected by western blot. Data are representative of the net responses observed in at least 4 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed to S. Typhi. Each dot is the average of 2 technical independent replicates. The density was normalized to the corresponding pan kinase. Two-tailed nested-t-tests were used to account for the repeated measures within the groups. Dotted lines represent the mean responses. (F) Shown is a representative immunoblot analysis of protein lysates. Results from uninfected media controls (UN) and acute and chronic strains are displayed (G) Correlation between phosphorylation levels of S6 and MAPK using the combined data after exposure to S. Typhi strains isolated from acutely and chronically infected individuals. The solid line represents the trendline. Dashed lines represent 95% confidence intervals. Shown are the coefficient of determination “r” and the “P” value. Correlations used the two-sided Pearson Product Moment tests. (H-J) MAPK regulated transcription factors (H) Phospho-ATF-2, (I) Phospho-MEF2, and (J) Phospho-c-Myc were detected by ELISA-based assays, TransAM assays. Data are representative of the net responses observed in 5 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed S. Typhi. Each dot is the average of 2 technical independent replicates. Two-tailed nested-t-test was used to account for the repeated measures within the groups. P values < 0.05 were considered statistically significant. Dotted lines represent the mean responses.