Fig. 3.

BMS-794833 inhibits osteosarcoma cell proliferation through the VEGFR/Ras/CDK2 axis. (A) U2OS and MG63 cells were treated with BMS-794833 at the shown concentrations (10 μM, 20 μM) for 24 h, and the expression levels of CDK2 protein and nucleic acid were detected by western blot assay and RT-PCR. (B) The volcano map showed the differential genes of the high and low expression CDK2 groups. (C) Venn diagram shows overlapping genes. DMS-794833 and DMSO were used to treat U2OS and MG63 cells transfected with shNC or shCDK2, and cell proliferation was measured by colony formation (D) and CCK8 (E). All experiments carried out in triplicate. *P < 0.05 and **P < 0.01 comparing with control group with t-Test in GraphPad Prism 8.0 software.