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. 1998 May;72(5):4080–4087. doi: 10.1128/jvi.72.5.4080-4087.1998

TABLE 1.

Ability of rVV expressing different levels of murine cytokines to protect mice against RSV challenge 3 weeks after vaccination

Vaccine Gene inserted at the following VV genome locus:
Titer of F proteina Cytokine expression (ng)b
Antibody response to RSVc Lung RSV titerd BAL cell counte
TK VP37 S/N C/A
VSCF F 759 ND ND 5.2 ± 0.3 <1.7h 5.8 ± 0.2i
VRBF F 1,995 ND ND 5.1 ± 0.4 <1.7h ND
VRBmIL-2 IL-2 ND 3,470 210 <1.5 4.4 ± 0.3 ND
VRBF.mIL-2low IL-2 F 1,738 5 1 4.5 ± 0.1g <1.7h ND
VSCF.mIL-2high F IL-2 708 3,400 310 4.1 ± 0.1f <1.7h 5.8 ± 0.1i
VRBmIL-4 IL-4 ND 8,650 840 <1.5 ND ND
VRBF.mIL-4low IL-4 F 2,884 7 1 5.1 ± 0.2 <1.7h ND
VSCF.mIL-4high F IL-4 776 6,700 490 5.0 ± 0.2 <1.7h 5.7 ± 0.1i
VRBmIFN-γ IFN-γ ND 3,430 315 <1.5 4.2 ± 0.2 ND
VRBF.mIFN-γlow IFN-γ F 1,548 BD BD 4.5 ± 0.2g <1.7h ND
VSCF.mIFN-γhigh F IFN-γ 794 3,630 300 3.0 ± 0.2f <1.7h 5.6 ± 0.1i
VA-βgal βgal ND ND ND <1.5 4.3 ± 0.2 5.2 ± 0.1
a

Means (n = 3) of titers of F glycoprotein determined by an ELISA. ND, not determined. 

b

Total cytokine levels in 60-mm-diameter petri dish. BD, below detection. S/N, supernatant; C/A, cell associated. 

c

Mean ± SD log10 titer of antibody to RSV determined by an ELISA 3 weeks postvaccination. 

d

Mean ± SD log10 PFU of RSV per gram recovered from lungs of mice 5 days after RSV challenge. 

e

Mean ± SD log10 cells per milliliter 5 days after RSV challenge. 

f

The probabilities of the differences in antibody responses between VSCF-vaccinated mice and VSCF.mIL-2- or VSCF.mIFN-γ-vaccinated mice were <0.02 and <0.0001, respectively, as calculated by Student’s t test. 

g

Antibody responses between VRBF-vaccinated mice and VRBF.mIL-2- or VRBF.mIFN-γ-vaccinated mice were significantly different, as determined by Student’s t test (P < 0.02 and P < 0.06, respectively). 

h

Virus titers between vaccinated and control mice were significantly different, as determined by Student’s t test (P < 0.0001). 

i

BAL cell counts in vaccinated mice were significantly different from those in VA-βgal-treated control mice, as determined by Student’s t test (P < 0.006). 

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