TABLE 3.
Peak levels of cytokine production from in vitro-restimulated immune splenocytesa
| Vaccine | Production (pg/ml) of:
|
||||
|---|---|---|---|---|---|
| IL-2 (72 h) | IL-4 | IL-5 | IL-10 (96 h) | IFN-γ (96 h) | |
| VSCF | 227 ± 6 | <15 | <10 | 2,423 ± 100 | 24,774 ± 695 |
| VV-F.mIL-2high | 163 ± 1 | <15 | <10 | 411 ± 10 | 3,241 ± 315 |
| VV-F.mIL-4high | 209 ± 6 | <15 | <10 | 2,273 ± 209 | 25,513 ± 1,413 |
| VV-F.mIFN-γhigh | 203 ± 10 | <15 | <10 | 314 ± 10 | 9,641 ± 362 |
| VA-βgal | 118 ± 10 | <15 | <10 | 78 ± 9 | 4,097 ± 174 |
a
Spleens were removed from mice 3 to 4 weeks after immunization with rVV and restimulated with gamma-irradiated RSV-infected (multiplicity of infection, 1) autologous splenocytes at a 5:1 effector cell/stimulator cell ratio. Supernatants were harvested on a daily basis and assessed for cytokines by an ELISA. Results are given as mean ± SD.