TABLE 4.
Analysis of BAL lymphocyte subsets 5 days after RSV challengea
Vaccineb | No. of cells/ml
(103)
|
|||
---|---|---|---|---|
CD4− CD8− | CD4+ | CD8+ | CD4+ CD8+ | |
VSCF | 493 | 535 | 779 | 0.7 |
VV-F.mIL-2high | 246 | 208 | 311 | 0.7 |
VV-F.mIL-4high | 378 | 330 | 453 | 0.7 |
VV-F.mIFN-γhigh | 360 | 200 | 407 | 0.5 |
VA-βgal | 105 | 198 | 178 | 1 |
Cells obtained from repeated BAL of groups of five mice were pooled, and total cells were counted by light microscopy. Cells were stained for CD4+ and CD8+ and analyzed by flow cytometry. The percentage of cells displaying CD4+ or CD8+ was converted into thousands of cells per group of five mice.
Mice were immunized i.p. with 2 × 106 PFU of rVV and challenged i.n. with RSV 3 weeks after vaccination.