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. 2024 Mar 25;14:7082. doi: 10.1038/s41598-024-57854-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cis elements in the FOXA1-binding regions of the SEMA3C locus. FOXA1 motif-containing regions of the SEMA3C promoter, second intron, and twelfth intron were cloned into the reporter vector, pGL3-Basic. LNCaP were co-transfected with reporter plasmids and either empty overexpression vector (‘pc’) or a FOXA1 overexpression plasmid (‘FOXA1’), stimulated with R1881 (5 nM), and then harvested to determine luciferase activity (a). Overexpression of FOXA1 was verified by Western blot. pGL3-S3Ci2 reporter construct was titrated against increasing amounts of FOXA1 overexpression plasmid (b). Western blot was used to monitor FOXA1 levels. Cloned regions are displayed and FOXA1 motifs (red) and AR elements (bold, underlined) are indicated (c). Minus strands are shown.