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N1 limit of detection in acid-pH and Direct Lysis Methods. Saliva samples were spiked with commercial N1 viral-like particles (VLPs) to control for the copy number concentrations. 10, 5, 2.5 and 1 copy/μL saliva was created, processed by Acid-pH and Direct Lysis Methods, and run with RT-qPCR to detect N1. The dashed line represents the detection limit. n = 5 PCR replicates per extraction.
