Table 1.
Phenotypic profiling of primary screening strains with twenty antibiotics.
| Drug class | Drug name |
K. pneumoniae Ecl8 |
K. pneumoniae NCTC 13438 |
A. baumannii NCTC 13424 |
|||
|---|---|---|---|---|---|---|---|
| MIC (mg/L) | Interp. | MIC (mg/L) | Interp. | MIC (mg/L) | Interp. | ||
| Aminoglycosides | Amikacin | 2 | S | 64 | R | >512 | R |
| Apramycina | 4 | Sc | 4 | Sc | 8 | Sc | |
| Gentamicin | 1 | S | 4 | R | >512 | R | |
| Tobramycin | 1 | S | 32 | R | >512 | R | |
| Monobactams | Aztreonam | 0.25 | S | >512 | R | 32 | ID |
| Extended-spectrum cephalosporins | Cefepime | 0.12 | S | 128 | R | 32 | R |
| Cefotaxime | 0.06 | S | 256 | R | 512 | R | |
| Ceftazidime | 0.5 | S | 512 | R | 64 | R | |
| Carbapenems | Imipenem | 0.25 | S | 128 | R | 128 | R |
| Meropenem | 0.06 | S | 128 | R | 64 | R | |
| Phosphonic acids | Fosfomycin | 64 | R | 16 | S | 64 | ID |
| Polymyxins | Colistin | 4 | Sb | 2 | Sb | 4 | Sb |
| Polymyxin B | 2 | Sc | 2 | Sc | 2 | Sc | |
| Phenicols | Chloramphenicol | 4 | S | 32 | R | 64 | ID |
| Fluoroquinolones | Ciprofloxacin | 0.12 | S | >512 | R | 128 | R |
| Levofloxacin | 0.12 | S | 128 | R | 32 | R | |
| DHFR inhibitors | Trimethoprim | 4 | S | >512 | R | 128 | R |
| Sulfonamides | Sulfamethoxazole | 64 | Rc | >512 | Rc | >512 | Rc |
| Tetracyclines | Minocycline | 2 | Sb | 4 | Sb | 4 | Sb |
| Tetracycline | 1 | Sb | 4 | Sb | 256 | Rb | |
MICs (minimum inhibitory concentrations) were determined using the microbroth dilution method from at least three independent biological replicates. There was no more than one two-fold dilution difference in MICs between biological replicates. MIC values indicated were the most frequently obtained between replicates.
HTS control.
S/R interpretation based on the EUCAST MIC distribution and epidemiological cut-off (ECOFFS) values.
No EUCAST breakpoint available for this drug-bacterial species combination; categorized “S” (sensitive) if ≤4-fold or “R” (resistant) if MIC ≥4-fold the MIC for the relevant susceptible EUCAST QC strain. ID, insufficient EUCAST data to interpret.