FIG. 4.
Effect of Vpr and/or MA NLS mutations on infectivity in dividing and nondividing HeLa-CD4-LTR/β-gal cells. (A and B) Dividing (A) or gamma-irradiated (4,000 rads) (B) HeLa-CD4/LTR-β-gal cells were analyzed by fluorescence-activated cell sorting as described in the legend to Fig. 3. (C and D) Dividing (C) or G2-growth-arrested (D) cells were infected with HIV-1 variants containing single or double mutations in the MA NLS and/or Vpr at the indicated MOI. Infectivity was assayed by transactivation of an endogenous LTR/β-gal gene as described previously (30), and histograms represent the mean numbers of X-Gal-positive cells from triplicate measurements from one experiment. Data are for wild-type (bars 1), Vpr− (bars 2), MA NLS− (bars 3), or Vpr− MA NLS− (bars 4) virus. For the infection at an MOI of 0.05 TCID50/cell, the data are representative of four independent experiments. Error bars represent standard deviations.