FIG. 7.

Densitometric analysis of the synthesis and nuclear translocation of viral DNA in G₂-growth-arrested MT4 or HeLa-CD4-LTR/β-gal cells infected with Vpr and/or MA NLS HIV-1 variants. (A and C) Densitometric analysis of the results presented in Fig. 6; data are for wild-type (bars 1), Vpr⁻ (bars 2), MA NLS⁻ (bars 3), or Vpr⁻ MA NLS⁻ (bars 4) virus. (B and D) Densitometric analysis of PCR products obtained following infection of gamma-irradiated HeLa-CD4-LTR/β-gal cells as described in the legend to Fig. 6. Cells were infected at an MOI of 0.05 TCID₅₀/cell with MA NLS⁻ viruses containing the indicated Vpr alleles.