Figure 7.

Activation of p53 (pSer392), expression of p21 and DNA damage analyses. Rat neonatal cardiomyocytes were incubated with dexrazoxane (DEX) (orange) and XK469 (blue) either alone or coincubated with 1.2 µM daunorubicin (DAU). The phosphorylated isoform of p53 protein (pSer392) (A, B), the expression of p21 mRNA (C, D), alkali-labile sites (E) and phosphorylation of gH2AX (F) were evaluated (see Supplementary Figures 5–8 for details). Statistical analyses: n = 4, mean ± SD, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “c”—compared with control, “d”—compared with daunorubicin.