Representative formulation scheme for preparing IL-incorporated LNPs.

Component	Molar ratio in final LNP mixture	Stock concentration (mg mL−1)	Volume of the stock solution required (μL)
Ethanolic phase
C12-200	50	1	12.5
Cholesterol	38.5	1	3.1
DSPC	10	0.1	16.9
Ethanol	—	—	92.5
Total ethanolic phase volume			125
Aqueous phase
siRNA	400 nM	1	2.5
1 : 1 or 1 : 2 IL	—	—	12.5/25/50
10 mM citrate buffer	—	—	q.s. to 125 μL
Nuclease-free water			250
Final volume of LNPs prepared (μL)			500