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. 2021 Jan 22;7(4):eabd9502. doi: 10.1126/sciadv.abd9502

Fig. 2. E-stim prevents IFN-γ–induced structural deterioration of myobundles.

Fig. 2

E-Stim prevents IFN-γ–induced structural deterioration of myobundles. (A to C) Representative myobundle cross sections immunostained for (A) F-actin (green; scale bars, 500 μm), (B) sarcomeric α-actinin (SAA; red; scale bars, 500 μm), and (C) dystrophin (Dys; red; scale bars, 25 μm). (D to G) Quantification of (D) myobundle cross-sectional area (CSA), (E) number of nuclei per cross section, (F) F-actin+ area, and (G) myotube diameter [n = 19 to 27 images from three donors per group, 3 to 5 myobundles for each donor for (D) to (F), n > 900 myotubes from three donors per group for (G)]. (H) Force amplitude normalized per myobundle CSA (specific force, n = 20 to 23 myobundles from three donors per group). *P < 0.05 versus IFN-γ−, #P < 0.05 versus IFN-γ+, **P < 0.05. NS, nonsignificant. Data are presented as means ± SEM.