Fig. 3. In vitro turnover number of ArM variants identified in the periplasmic screening.

For each reaction, the three most active variants identified in the whole-cell screening were purified, and their TON was determined. Reactions were carried out at 37°C and 200 rpm for 20 hours. Bars represent mean TONs of technical triplicate reactions with SD as error bars and individual replicates as circles. For comparison, the free cofactor and wild-type Sav variant (wt) were included. Mutants are designated by the amino acids in positions 112 and 121.