TABLE 1.
Overall mutation frequencies
RT | No. of plaques screened | No. of mutants | Mutation frequencya (10−3) |
---|---|---|---|
Wild type | 13,662 | 138 | 8.60 |
E89G | 13,916 | 108 | 6.26 |
M184V | 14,804 | 104 | 5.53 |
E89G/M184V | 7,173 | 99 | 12.30 |
Corrected for background by subtracting the spontaneous background frequency of 1.50 × 10−3 from the frequency observed for a given RT. Spontaneous background was measured by using uncopied gapped M13mp2 DNA.