TABLE 2.

Error rates at selected mutational hotspots

Site	Wild type		E89G		M184V		E89G/M184V
	No. of errors	Error rate	No. of errors	Error rate	No. of errors	Error rate	No. of errors	Error rate
Base substitutions
−36	26	1/370	35	1/296	37	1/305	55	1/87.5
−35	8	1/1,203	0	CDd	1	1/11,284	0	CD
84	5	1/1,926	0	CD	1	1/11,284	0	CD
89	5	1/1,926	4	1/2,588	5	1/2,257	1	1/4,829
112	5	1/1,926	0a	CD	0a	CD	0a	CD
145	3	1/3,209	1	1/10,351	5	1/2,257	2	1/2,415
Frameshifts
−34 to −36	0b	CD	23b	1/1,350	0b	CD	12b	1/1,207
70 to 73	5	1/7,702	2	1/20,702	0	CD	6	1/3,220
88 to 90	5	1/5,777	1	1/31,054	0	CD	2	1/7,244
106 to 108	6	1/4,814	1c	1/31,054	1c	1/33,852	1c	1/14,488
137 to 139	9	1/3,209	7	1/4,436	16	1/2,116	10	1/1,449
Overall	138	1/17,651	108	1/24,249	104	1/27,450	99	1/12,341

^aIn comparison of each mutant RT to wild type, P values ranged from 0.069 to 0.076. 

^bIn comparison of E89G to wild type or M184V as well as in comparison of E89G/M184V to wild type or M184V, P values were <0.0001 each. 

^cIn comparison of each mutant RT to wild type, P values ranged from 0.139 to 0.244. 

^dCD, cannot determine.