Table 3. IC50 Values for Growth Factor Receptor Inhibitors, Downstream Signaling Molecules Inhibitors and Cytotoxic Agents.
| IC50 value (µM) |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ponatinib (Abl/ PDGFRa/VEGFR2/FGFR1 inhibitor) |
Entrectinib (TrkA/B/C/ROS/ALK inhibitor) |
AZD4547 (FGFR 1/2/3 inhibitor) |
Trametinib (MEK 1/2 inhibitor) |
Selumetinib (MEK/ERK1/ERK2 inhibitor) |
Miransertib (AKT1/2/3 inhibitor) |
Lorlatinib (ALK/Ros1 inhibitor) |
Docetaxel (inhibits of depolymerization of microtubules) |
Paclitaxel (microtubule polymer stabilizer) |
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| FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | FBS 2% | FBS 10% | |
| HGC-27 | 0.37 ± 0.03 | 1.02 ± 0.18 | 0.71 ± 0.03 | 2.46 ± 0.54 | 2.90 ± 0.45 | > 10.00 | 8.13 ± 1.63 | > 10.00 | > 10.00 | > 10.00 | 0.08 ± 0.001 | 0.81 ± 0.55 | > 10.00 | > 10.00 | 0.0001 ± 0.05 | 0.003 ± 0.25 | 0.07 ± 0.006 | 0.08 ± 0.004 |
| AGS | 0.39 ± 0.03 | 0.85 ± 0.01 | 1.05 ± 0.17 | 2.73 ± 0.16 | 8.53 ± 2.57 | > 10.00 | 0.005 ± 0.001 | 0.002 ± 0.00 | 0.05 ± 0.01 | 1.53 ± 2.47 | 0.60 ± 0.06 | 0.39 ± 0.12 | 8.34 ± 4.79 | > 10.00 | 0.0001 ± 0.01 | 0.003 ± 0.06 | 0.15 ± 0.001 | 0.12 ± 0.02 |
| FU97 | 0.015 ± 0.002 | 0.05 ± 0.003 | 0.06 ± 0.02 | 0.26 ± 0.09 | 0.04 ± 0.007 | 0.08 ± 0.002 | 0.03 ± 0.006 | 0.005 ± 0.001 | 0.16 ± 0.03 | 0.30 ± 0.03 | 0.017 ± 0.004 | 0.07 ± 0.005 | 4.56 ± 2.30 | > 10.00 | 0.009 ± 0.36 | 0.002 ± 0.11 | 0.04 ± 0.004 | 0.06 ± 0.003 |
| MKN1 | 0.09 ± 0.04 | 0.74 ± 0.07 | 0.62 ± 0.11 | 4.18 ± 0.47 | 4.20 ± 2.91 | > 10.00 | 0.006 ± 0.001 | 0.014 ± 0.001 | 0.07 ± 0.08 | 1.99 ± 0.52 | 0.15 ± 0.03 | 0.75 ± 0.75 | > 10.00 | > 10.00 | 0.001 ± 0.001 | 0.002 ± 0.48 | 0.004 ± 0.12 | 0.007 ± 0.001 |
| MKN74 | 1.02 ± 0.20 | 2.12 ± 0.03 | 1.24 ± 0.06 | 2.55 ± 0.31 | 6.07 ± 0.64 | > 10.00 | 0.008 ± 0.008 | 1.35 ± 1.06 | 6.80 ± 0.16 | > 10.00 | 3.51 ± 0.73 | 7.27 ± 0.96 | > 10.00 | > 10.00 | 0.0001 ± 0.001 | 0.0004 ± 0.001 | 0.06 ± 0.001 | 0.07 ± 0.001 |
| NCI-N87 | 0.34 ± 0.06 | 1.03 ± 0.02 | 0.39 ± 0.18 | 2.11 ± 0.20 | 3.40 ± 1.22 | 7.27 ± 1.84 | 0.003 ± 0.001 | 0.03 ± 0.003 | 0.52 ± 0.03 | 0.47 ± 0.20 | 0.30 ± 0.05 | 1.69 ± 0.35 | > 10.00 | > 10.00 | 0.008 ± 0.19 | 0.001 ± 0.001 | 0.005 ± 0.26 | 0.001 ± 0.001 |
TKI: tyrosine kinase inhibitor; FBS: fetal bovine serum; IC50: 50% inhibitory concentration; EGFR: epidermal growth factor receptor; ALK anaplastic lymphoma kinase; CDK: cyclin dependent kinase; PDGFR: platelet-derived growth factor receptor; VEGFR: vascular endothelial growth factor receptor; FGFR: fibroblast growth factor receptor.