Extended Data Fig. 9. PD-L2 expression and protein levels increase in primary cultures from human patients upon establishment of cellular senescence.
(a) Gating strategy for detection of PD-L2+ cells in Fig. 5e and in the following panels. (b-c) SABG staining, p21 mRNA levels, PD-L2 mRNA levels, and PD-L2 protein levels measured by FACS with and without bleomycin treatment in (b) human endometrial cancer primary culture from patient VHIO-35035 and (c) human melanoma primary culture from patient VHIO-088. N = 4 independent experiments for gene expression, n = 1 for FACS. Data are presented as mean ± SEM. Two-sided t-tests were applied. Scale bars = 50 μm.