Fig. 2. A combination of PD-L2 ablation and chemotherapy results in CD8 T cell-dependent tumor remission.
a, Quantification of tumor growth for PD-L2 WT and KO Panc02 orthotopic tumors, untreated or treated with doxorubicin (4 mg kg−1) at days 7, 10 and 24. b, Representative images. PBS-injected groups (n = 9 mice); doxorubicin-treated groups (n = 10). c, Survival curve for the mice from a. d, Quantification and representative images of tumor growth for PD-L2 KO Panc02 tumors after doxorubicin treatment (4 mg kg−1, days 7 and 10) and repeated injections with IgG isotype control, anti-CD4 or anti-CD8 blocking antibodies (100 μg per injection) from day 3 after tumor cell injection and repeated every 3 days. Inverted red triangles indicate day of doxorubicin treatment. Luminescence units are photons (p) s−1 in the graphs and p s cm−2 steradian in the images. A two-way ANOVA and one-way ANOVA with Tukey post-hoc test were used.