Extended Data Fig. 4. A functional adaptive immune system is required for clearance of PD-L2-KO tumors.

(a) Quantification and representative images of Panc02 WT and PD-L2-KO tumors in nude mice, untreated and treated with doxorubicin on days 7 and 10. Luminescence units are photon/sec/cm²/stereoradian in the images. N = 3 for PD-L2-WT + PBS mice, n = 5 for PD-L2-KO + PBS, n = 6 for both doxo-treated groups. (b) Gating strategy to define circulating CD4⁺ and CD8⁺ T cell populations. (c) Percentage of CD4⁺ and CD8⁺ T cells among total CD45⁺ CD3⁺ cells in blood of mice after treatment with blocking anti-CD4 and anti-CD8 antibodies. N = 6-7 mice. Data are presented as mean ± SEM.

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