Fig. 3.

The effects of simvastatin (sim; 5 µM) and amfenac (0.1 µM) on the levels of active NF-κB p65 (a) and PGE2 (b) in ARPE-19 cells upon IL-1α (10 pg/ml) exposure. NF-κB results were normalized to the protein level of each sample and compared to the mean of DMSO group (D; 0.15%). PGE2 levels were measured from cell lysates as pg/ml values and compared to the mean of DMSO-treated control cells. Data were collected from 3 independent experiments including 2–3 samples per group in each experiment (total n = 8), and results are presented as mean ± SEM. *P < 0.05, ns—not significant by Mann–Whitney U test. [Kruskal–Wallis test was performed but not presented in the Figure, ***P < 0.001 (a), ns—not significant (b)]