Table 2.

Panel of monoclonal antibody reagents (with clones and commercial source) used for the immunophenotypic characterization of peripheral NK cell subsets

Fluorochrome
Tube	V500	PacB	FITC	PE	PC5	PC7	APC	APCH7
1	CD3 SP34-2 BD Pharmingen	CD8 RPA-T8 BD	CD27 M-T271 BD Pharmingen	CCR5 G155-178 BD Pharmingen	CD56 N901 Beckman–Coulter	CD45RO UCHL1 Beckman–Coulter	CD45RA HI100 BD	CD4 SK3 BD
Tube	PacO	PacB	FITC	PE	PercP	PC7	APC	APCH7
2	CD45 HI30 Invitrogen	HLA-DR L243 BioLegend	CD16 3G8 BD Pharmingen	CD54 LB-2 BD	CD11c N418 BioLegend	CD33 D3HL60.251 Beckman–Coulter	CD123 9F5 BD	CD14 MϕP9 BD

Immunofluorescence staining after in vitro activation
Tube	V500	PacB	FITC		PC5
3	CD3 SP34-2 BD Pharmingen	CD8 B9.11 Beckman Coulter	cyTNFα AAb11 BD Pharmingen		CD56 N901 Beckman Coulter
Tube	V500	PacB	FITC		PC5
4	CD3 SP34-2 BD Pharmingen	CD8 B9.11 Beckman Coulter	cyIFNγ 4S.B3 BD Pharmingen		CD56 N901 Beckman Coulter

The analysis of these cells was integrated in a panel where other subpopulations were also analysed.

BD Pharmingen (San Diego, CA, USA), Biolegend (San Diego, CA, USA), BD (Becton Dickinson Biosciences, San Jose, CA, USA), Beckman Coulter (Miami, FL, USA), Invitrogen (Life Technologies, Carlsbad, CA, USA); APC, Allophycocyanin; APCH7, allophycocyanin-hilite7; FITC, Fluorescein isothiocyanate; PacB, Pacific blue; PE, Phycoerythrin; PC5, Phycoerythrin–cyanine 5; PC7, Phycoerythrin–cyanine 7; Perc-P, Peridinin chlorophyll protein