Table 5.

Current status of the DNT IVB

Key event	Assay name	Cell type	Link to OECD appendix
Cell migration	UKN2 assay (cMINC)	hiPSC-derived NCCs	https://www.oecd.org/env/ehs/testing/appendix-b3-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Neurite outgrowth (central)	UKN4 assay (NeuriTox)	LUHMES cells (differentiated into morphologically and biochemically mature dopamine-like neurons)	https://www.oecd.org/env/ehs/testing/appendix-b4-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Neurite outgrowth (peripheral)	UKN5 assay (PeriTox)	hiPSC-derived iDRG	https://www.oecd.org/env/ehs/testing/appendix-b5-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Precursor proliferation	Neurosphere Assay NPC1	hNPCs	https://www.oecd.org/chemicalsafety/testing/appendix-ba-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Radial glia migration	Neurosphere assay NPC2a	hNPCs	https://www.oecd.org/chemicalsafety/testing/appendix-b2-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Neuronal migration	Neurosphere assay NPC2b	hNPCs
Oligodendrocyte migration	Neurosphere assay NPC2c	hNPCs
Neuronal differentiation	Neurosphere assay NPC3	hNPCs
Neurite outgrowth	Neurosphere assay NPC4	hNPCs
Oligodendrocyte differentiation	Neurosphere assay NPC5	hNPCs
Neurite outgrowth	Cortical initiation	Rat primary cortical neurons	https://www.oecd.org/chemicalsafety/testing/appendix-b6-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Neurite maturation	Cortical maturation	Rat primary cortical neurons	https://www.oecd.org/chemicalsafety/testing/appendix-b7-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Synaptogenesis	Cortical synapto	Rat primary cortical neurons
Network formation assay (NFA)	Cortical MEA	Rat primary cortical neurons	https://www.oecd.org/chemicalsafety/testing/appendix-b8-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Neurite outgrowth	hN initiation	hiPSC-derived neurons	https://www.oecd.org/chemicalsafety/testing/appendix-b9-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Proliferation, cytotoxicity and apoptosis assay	hNP1 Apop	hNPCs	https://www.oecd.org/chemicalsafety/testing/appendix-b10-guidance-evaluation-of-data-developmental-neurotoxicity-in-vitro-testing-battery.pdf (accessed on Nov 2, 2023)
Proliferation, cytotoxicity and apoptosis assay	hNP1 Prolif	hNPCs

cMINC circular migration inhibition of neural crest cells, NCCs neural crest cells, hiPSC human-induced pluripotent stem cell, iDRG immature dorsal root ganglia neurons, hNPCs human neural progenitor cells, LUHMES Lund human mesencephalic