Table 3.

Oligonucleotide sequences used in this study.

Target gene	Oligonucleotide sequence (5′–3′)	Products (bp)	Tm ºC	Reference
HrpE	F: GTCGACAAATGGAATTATTACCG R: CTCGAATTACTGGCCAACGGCTG	282	61	27
pHEN	F: GCCGCTGGATTGTTATTACCTCT R: CTATGCGGCCCCATTCA	850	54	This work
ScFv	F: ATCCTTCGCAAGACCCTTCCTCT R: AGAGAGAGATAGATTTGTAGAGA	900	57	18