Table 2.
Growth parameters of Pseudomonas putida EM42 mutant strains in batch cultures with D-glucose or D-xylose as a sole carbon source
| Strain and carbon source | µ max (h−1)1 | YX/S (gCDW gS −1)2 | qS (mmolS gCDW−1 h−1)2 | Lag phase (h)1 |
|---|---|---|---|---|
| EM42Δgcd xylABE+ XYL | 0.09 ± 0.00 | n.d. | n.d. | 41.94 ± 0.72 |
| PD310 GLU | 0.46 ± 0.01 | 0.38 ± 0.02 | 6.33 ± 0.42 | 1.40 ± 0.14 |
| PD310 XYL | 0.11 ± 0.00 | 0.31 ± 0.03 | 1.98 ± 0.32 | 10.04 ± 0.53 |
| PD506 XYL | 0.07 ± 0.00 | n.d. | n.d. | 31.72 ± 0.64 |
| PD584 XYL | 0.13 ± 0.00 | 0.35 ± 0.01 | 2.22 ± 0.16 | 4.60 ± 0.44 |
| PD689 XYL | 0.10 ± 0.00 | 0.31 ± 0.02 | 1.14 ± 0.06 | 18.25 ± 1.28 |
| PD584 L3 XYL | 0.21 ± 0.01 | 0.41 ± 0.07 | 3.07 ± 0.46 | 3.40 ± 0.60 |
| PD689 tt L1 XYL | 0.21 ± 0.00 | 0.37 ± 0.02 | 3.86 ± 0.10 | 3.70 ± 0.35 |
| PD855 | 0.20 ± 0.00 | 0.35 ± 0.03 | 3.46 ± 0.37 | 4.94 ± 0.39 |
GLU D-glucose, XYL D-xylose, n.d. not determined.
1The maximal specific growth rate µ max and growth lag were calculated from growth data obtained from cultures in 48-well plates using the deODorizer program80. Presented values are means from six biological replicates ± standard deviation.
2The biomass yield (YX/S) and specific carbon uptake rate (qS) were calculated from data obtained from cultures in Erlenmeyer flasks. Shown values are means from three biological replicates ± standard deviation.
Source data are provided as a Source Data file.