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. 2024 Mar 26;15:2652. doi: 10.1038/s41467-024-46828-1

Fig. 6. The entire SNARE motif of tomosyn contributes to its binding to the template complex.

Fig. 6

a Effects of tomosyn truncation or VAMP2 on the template complex. The left panel shows the schematic diagram of different tomosyn domains that remained in the truncated tomosyn relative to the entire SNARE domain, including the N-terminal SNARE motif or domain (NTD), the C-terminal SNARE domain (CTD), and the polybasic domain (PB). The right panel is the corresponding extension-time trajectory at constant mean force F in the presence of 2 μM free WT or truncated tomosyn or VAMP2 in the solution. The folding states corresponding to different extensions are labeled on the right and illustrated in Fig. 5c. The red curves represent the idealized transitions derived from hidden Markov modeling. b Left panel: the structure of the template complex (PDB ID 1SFC) with the mutated C-terminal hydrophobic layers indicated. Right panel: the extension-time trajectories at constant mean force showing folding and unfolding transition of the templated complex in the absence and presence of the tomosyn SNARE motif. See also Supplementary Fig. 10.