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. 2024 Mar 26;15(3):235. doi: 10.1038/s41419-024-06624-7

Fig. 2. USP3 promotes the proliferation, migration and invasion of OS.

Fig. 2

A The changes in protein expression after knockdown or overexpression of USP3 were detected by WB. B Changes in mRNA expression after knockdown or overexpression of USP3. C The effect of knockdown or overexpression of USP3 on the proliferation of OS cells was detected by CCK-8. D The effect of knockdown or overexpression of USP3 on the proliferation of OS cells was detected by colony formation assay, and the quantitative analysis is presented in a histogram. E The effect of knockdown or overexpression of USP3 on the migration activity of OS cells was detected by a migration assay (scale bar, 50 µm), and the quantitative data are presented in a histogram. F The effect of knockdown or overexpression of USP3 on the invasion activity of OS cells was detected by a Matrigel invasion assay (scale bar, 50 µm), and the quantitative data are presented in a histogram. (Data from three independent experiments. *P < 0.05; **P < 0.01; sh-NC: OS cells transfected with a negative lentiviral vector; sh-USP3: OS cells transfected with lentiviral vector-shRNA downregulating USP3; OE-Con OS cells transfected with a negative lentiviral vector; OE-USP3 OS cells transfected with lentiviral vector-USP3 upregulating USP3).