Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 26;15(3):235. doi: 10.1038/s41419-024-06624-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A 143B and HOS cells coexpressed HA-ubiquitin, Flag-EPHA2 and Myc-USP3. EPHA2 ubiquitination was then analyzed by WB. B 143B and HOS cells transfected with control (sh-NC) or USP3 knockdown (sh-USP3) plasmids were immunoprecipitated, and EPHA2 ubiquitination was then detected by Western blotting. C HEK293T cells transfected with HA-Ubi, Myc-USP3, Flag-EPHA2 or one of four site-specific mutants of EPHA2 (K702R, K778R, K882R and K945R) were treated with 10 µM MG132 for 6 h, and the ubiquitination of the EPHA2 DBD domain was then analyzed.