Fig. 6. USP3 promotes the proliferation, migration and invasion of OS by regulating EPHA2 and activating the PI3K/AKT signaling pathway.

A OS cells were transfected with USP3 overexpression lentivirus (OE-USP3) and EPHA2 knockdown lentivirus (sh-EPHA2). Control shRNA lentivirus was used to establish the control stable cell line (Mock). Knockdown of EPHA2 partially reversed the activation effect of USP3 on the PI3K/AKT signaling pathway. B The effect of EPHA2 knockdown on the proliferation of OS cells overexpressing USP3 was detected by CCK8. C The cell colony formation ability of OS cells in the mock+sh-NC, mock+sh-EPHA2, sh-EPHA2 + OE-USP3 or sh-NC + OE-USP3 groups was assessed by coloning formation assay. D The cell migration ability of OS cells in the mock+sh-NC, mock+sh-EPHA2, sh-EPHA2 + OE-USP3 or sh-NC + OE-USP3 groups was assessed by a Matrigel chamber assay. E The cell invasion ability of OS cells in the mock+sh-NC, mock+sh-EPHA2, sh-EPHA2 + OE-USP3 or sh-NC + OE-USP3 groups was assessed by a Matrigel invasion chamber assay. All values shown are the means ± SDs of triplicate measurements, and the experiments were repeated 3 times with similar results. The quantitative analysis is presented in the histogram shown in the right. *P < 0.05; **P < 0.01.