Table 3.
Sequencing methods of RNA modifications
| Technologies | Year | Resolution | Description | Ref. |
|---|---|---|---|---|
| MeRIP-seq (m6A-seq) | 2012 | 100-200nt | m6A-specific sequencing method based on antibody-mediated capture and massively parallel sequencing | 442 |
| miCLIP | 2015 | single nucleotide | individual nucleotide resolution cross-linking and immunoprecipitation method for m6A and m6Am | 443 |
| PA-m6A-seq | 2015 | 23nt | m6A sequencing assisted by photo-crosslinking | 444 |
| m6A-REF-seq | 2019 | single nucleotide | antibody-independent m6A mapping based on the m6A-sensitive RNA endoribonuclease | 445 |
| DART-seq | 2019 | single nucleotide | an antibody-free method for m6A targeting deamination adjacent to modification sites | 446 |
| m6ACE-seq | 2019 | single nucleotide | m6A cross-linking exonuclease sequencing method | 447 |
| m6A-SEAL-seq | 2020 | single nucleotide | FTO-assisted m6A selective chemical labeling method | 448 |
| m6A-label-seq | 2020 | single nucleotide | a metabolic labeling method for m6A | 449 |
| m1A-seq | 2016 | 50-200nt | a protocol for mapping m1A at single-nucleotide resolution | 101 |
| m1A-ID-seq | 2016 | \ | a m1A profiling method based on immunoprecipitation and the inherent ability of m1A to stall reverse transcription | 450 |
| m1A-MAP | 2017 | single nucleotide | a misincorporation- assisted profiling method for m1A | 451 |
| Aza-IP | 2013 | \ | 5-azacytidine-mediated RNAimmunoprecipitation | 452 |
| Bisulfite sequencing | 2017 | single nucleotide | a RNA bisulfite sequencing method of m5C | 453 |
| m5C-RIP-seq | 2017 | \ | a m5C profiling using RNA immunoprecipitation followed by a deep sequencing | 454 |
| Pseudo-seq | 2014 | single nucleotide | a genome-wide, single-nucleotide-resolution method for pseudouridine | 167 |
| Ψ-seq/Psi-seq | 2014 | single nucleotide | a protocol for transcriptome-wide quantitative mapping of Ψ | 455 |
| CeU-seq | 2015 | single nucleotide | N3-CMC–enriched Ψ sequencing method | 456 |
| m7G-MeRIP-Seq | 2019 | 100-200nt | m7G-methylated immunoprecipitation sequencing method | 154 |
| m7G-MaP-seq | 2019 | single nucleotide | high-throughput m7G mutational profiling sequencing | 457 |
| m7G-miCLIP-Seq | 2019 | single nucleotide | m7G individual-nucleotide-resolution cross-linking and immunoprecipitation with sequencing method | 458 |
| ac4C-RIP-seq | 2018 | \ | transcriptome-wide ac4C-targeted RNA immunoprecipitation sequencing | 134 |
| ac4C-seq | 2021 | single nucleotide | a protocol for the quantitative single-nucleotide resolution mapping of ac4C | 459 |
| ICE-seq | 2011 | \ | inosine chemical erasing method with deep sequencing method | 460 |