Western blot analyses of HeLa CD4 pools transfected with vectors expressing either the F12-HIV gag, pol, tat, vpu, nef, or vif gene or transduced with rev-expressing retrovirus vector. Hygromycin-resistant HeLa CD4 cells (C−) and parental Hut78/F12 cells were used as negative and positive controls, respectively. Molecular size markers (in kilodaltons) are given to the left of each panel.