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RIPA of HeLa CD4 pools transfected with env (I)- or vpr (II)-expressing vectors. Hygromycin-resistant HeLa CD4 cells (Cā) and Hut-78/F12 cells were used as negative and positive controls, respectively. Cell lysates were incubated with normal (ā) or hyperimmune (+) specific rabbit antisera. Molecular size markers (in kilodaltons) are given to the left of each panel.
