FIG. 8.

RT activities in supernatants of CEMss cells transduced with retrovirus vectors expressing F12-HIV Vif or Nef protein at different days after infection (p.i.) (MOI of 1) with three different T-tropic HIV-1 clinical isolates. As a control, CEMss cells transduced with the empty L-NGFr retrovirus vector were also infected. A total of 10⁶ cells were incubated for 24 h with the appropriate volumes of supernatants containing the challenging virus and were then washed and resuspended in fresh medium. Data from a representative of two different experiments are reported.