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Reconstitution of NLRP3-deficient U937 with doxycycline-inducible NLRP3 variants. NLRP3-deficient U937 reconstituted with doxycycline-inducible WT NLRP3 (pInd-WT), indicated NLRP3 variants, and the empty vector (pInd) were treated with doxycycline (indicated doses, 4 h). U937 treated with LPS (40 ng/ml, 3 h) was used as a control. NLRP3 protein levels were analyzed by WB. Source data are available for this figure: SourceData FS1.
